2nd Bachelor in Medical Biology - Cytology

Histological techniques and laboratory

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ECTS Credits3
Number of Hours (h/year)60
Teaching staffHEMELAERS, Laurent
Course UnitCompulsory
TermQuad. 1 and 2

Know how to use optical microscopy, to make dilutions, to weigh…( basic chemical procedures). 

Background of biology, organic chemistry and histology ( 1st year).

Aims and Objectives

To be able to integrate in pathological-anatomy laboratory.

To be able to use microtomy.

to be able to use cytologic specimens



Use of microtomes and cryostats

Stains of connective fibers, carbohydrates, proteins and nucleic acids.

Methods for demonstrating: pigments and precipitate; microorganisms; enzymatic activities.

Quality control in histological techniques.

Electron microscopy.

Immuno histochemistry.

Nervous tissue stain.

Cytology staining

Practical activities (collection of specimens, fixation, inclusion, embedding, sections; histological, histochemical and enzymatic stains).



Bancroft J., Gamble M., Theory and practice of histological techniques, Chruchill Livingstone, 2005, 796 p

Fortier J, Hould R., Histotechnologie, théorie et procédés, CCDMD, 2003, 717 p

Carson F., Histotechnology, a self-instructional text, ASCP Press, 1997, 304 p

Gabe M., Techniques histologiques, Masson, 1968

Stevens A., Lowe J., Histologie humaine, De Boeck Université, 1997, 536 p

Histotechnology, a self-intructional text, Freida L. Carson, ASCP Press, 1997

Teaching and Learning MethodsLectures
Assessment MethodsWritten examinations
Projects or practical work
Continuous assessment

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