2nd Bachelor in Medical Biology - Cytology

Biochemistry laboratory

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ECTS Credits5
Number of Hours (h/year)95
Teaching staffFRANCK, Marianne
Course UnitCompulsory
TermQuad. 1 and 2

Good grounding in theoretical course and statistics ; capacity for laboratory manipulations.

Aims and Objectives

To approach the theoretical knowledge of biochemical technology ; to acquire the know-how; to learn experimental work planning and laboratory apparatus experience; to acquire experimental precision and to examine the results critically.


Visible and U.V. light spectroscopy principles : theory ( Beer-Lambert’s law ; absorption spectrum) ; manipulations (absorption spectrum and molar extinction coefficient determination, proteins and hemoglobin assays).

Enzymology : enzymatic kinetics ( KM and VM determinations; inhibition ); enzymatic activity assays ( optimum conditions; various factors influence).

Separation technology : electrophoresis; ion exchange chromatography.

Applied in medical chemistry assays : blood glucose; nitrogen metabolism ( total urinary nitrogen by Kjeldahl method, urea, creatinine, uric acid); total bilirubin; acid-base balance ( blood gas analyser).


PLUMMER D.T., Introduction aux techniques de Biochimie, Ed. McGraw-Hill, Paris, 1989

AUDIGIE Cl., DUPONT G., ZONSZAIN F., Principes des méthodes d'analyse biochimique, t. 1 et 2, 4e Ed., Ed. Doin, Paris, 1993

GAVRILOVIC M., MAGINOT M.J., SCHWARTZ-GAVRILOVIC L., WALLACH J., Manipulations d'analyse biochimique, 3e Ed., Ed. Doin, Paris, 1996

Teaching and Learning MethodsLaboratory work

Tutorials and exercices

Assessment MethodsWritten examinations
Projects or practical work
Continuous assessment

Examens écrit et pratqiue.


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