3rd Bachelor in Medical Biology - Clinical Chemistry

Toxicology : therapeutic control techniques

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ECTS Credits2
Number of Hours (h/year)30
Teaching staffDUBOIS, Nathalie
Course UnitCompulsory
TermQuad. 1 and 2
CodePARA-D_GBMCC0005/3
Prerequisite

General notions of analytical chemistry and physics.

Aims and Objectives

To understand the importance of therapeutic control and why it is essential to select the good analytical procedure or to know the limits of used methods.

To know the methods more utilized in the laboratories, their advantages and their disadvantages.

To know the principal solvents used in professional or household surrounding. To understand their toxicity and to identify the signs of that toxicity.

To understand the determinations of solvents and their metabolites assays.

Description

Generalities on therapeutic control : definition and useful purpose ; medicine drugs accessible to therapeutic monitoring ; when take a sample ?

Interpretation of therapeutic control result: calculus of pharmacokinetic control result: calculus of pharmacokinetic parameters; adjustement of daily posologies on basis therapeutic control; problem with free forms of medicine drugs.

Assays of medicine drugs :

· Immunological techniques (ELISA, EMIT, FPIA, RIA…)

· Choice of monoclonal and polyclonal antibodies

· Dose-response relationship

· Standard separating techniques (GC, LC and different detectors)

· Methods based on emission and/or absorption

· New separating techniques (ICP, RMN, TOF...)

· Advantages and disadvantages of separating and immunological techniques.

Analytic validation

Medicine drugs no accessible to therapeutic control : indirect control.

Solvents toxicity : poisons penetration in the organism ; physico-chemical properties of solvents ; toxicity signs and symbols ; organic solvents .

Methods : blood assays of solvents and urinary assays of metabolites.

Bibliography

ROSSET R., CAUDE M. et JARDY A., Manuel pratique de chromatographie en phase liquide, 2e Ed.; Masson, Paris; 1982

CONSTANTIN E., Spectrométrie de masse : principes et applications, 2e Ed., Edition TEC et DOC, Paris, 1996

GERHARDS P., BONS U., SAWASAKI J. et al., GC/MS in Clinical Chemistry, Edition Wiley-VCH, Weinheim, 1999

KINTZ P., éditeur, Toxicologie et Pharmacologie Médico-légale, Edition Elsevier, 2001.

Teaching and Learning MethodsLectures
Assessment MethodsOral examinations
NotesYes
LanguageFrench

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