2nd Bachelor in Medical Biology - Clinical Chemistry
Biochemistry laboratory
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ECTS Credits | 5 |
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Number of Hours (h/year) | 95 |
Teaching staff | FRANCK, Marianne |
Course Unit | Compulsory |
Term | Quad. 1 and 2 |
Code | PARA-D_GBM0002/2 |
Prerequisite | Good grounding in theoretical course and statistics ; capacity for laboratory manipulations. |
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Aims and Objectives | To approach the theoretical knowledge of biochemical technology ; to acquire the know-how; to learn experimental work planning and laboratory apparatus experience; to acquire experimental precision and to examine the results critically. |
Description | Visible and U.V. light spectroscopy principles : theory ( Beer-Lambert’s law ; absorption spectrum) ; manipulations (absorption spectrum and molar extinction coefficient determination, proteins and hemoglobin assays). Enzymology : enzymatic kinetics ( KM and VM determinations; inhibition ); enzymatic activity assays ( optimum conditions; various factors influence). Separation technology : electrophoresis; ion exchange chromatography. Applied in medical chemistry assays : blood glucose; nitrogen metabolism ( total urinary nitrogen by Kjeldahl method, urea, creatinine, uric acid); total bilirubin; acid-base balance ( blood gas analyser). |
Bibliography | PLUMMER D.T., Introduction aux techniques de Biochimie, Ed. McGraw-Hill, Paris, 1989 AUDIGIE Cl., DUPONT G., ZONSZAIN F., Principes des méthodes d'analyse biochimique, t. 1 et 2, 4e Ed., Ed. Doin, Paris, 1993 GAVRILOVIC M., MAGINOT M.J., SCHWARTZ-GAVRILOVIC L., WALLACH J., Manipulations d'analyse biochimique, 3e Ed., Ed. Doin, Paris, 1996 |
Teaching and Learning Methods | Laboratory work Tutorials and exercices |
Assessment Methods | Written examinations Projects or practical work Continuous assessment Examens écrit et pratqiue. |
Notes | Yes |
Language | French |
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